ANTHONY JOHN ATALA, MD
Urology at Charlois Blvd, Winston Salem, NC

2012008, Apr 12, 2012

Oct 6, 2011

13/267448

Hyun-Wook Kang - Winston-Salem NC, US
Sang Jin Lee - Winston-Salem NC, US
Anthony Atala - Winston-Salem NC, US
James J. Yoo - Winston-Salem NC, US

A61F 2/02, B05C 11/00, B05D 1/00

623 2372, 427 231, 118695

A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

2008030, Dec 11, 2008

Jun 6, 2008

12/134813

Anthony Atala - Winston-Salem NC, US
James J. Yoo - Winston-Salem NC, US

Wake Forest University Health Sciences, - Winston-Salem NC

A61F 2/00, C12N 5/06, A61P 7/00, A61K 35/12

424423, 435369, 424 937, 435378

Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro. The kidney cells may include peritubular interstitial cells of the kidney, and preferably produce erythropoietin (EPO). The kidney cells may also be selected based upon EPO production. Methods of producing an isolated population of EPO producing cells are also provided, and methods of treating a kidney disease resulting in decreased EPO production in a patient in need thereof are provided, including administering the population to the patient, whereby the cells produce EPO in vivo.

2006025, Nov 9, 2006

Mar 10, 2006

11/373066

Anthony Atala - Winston Salem NC, US
James Yoo - Winston Salem NC, US

Wake Forest University Health Sciences - Winston-Salem NC

A61F 2/24, C12N 5/08

623002130, 435401000, 623915000

The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.

2011005, Mar 10, 2011

Sep 8, 2010

12/877282

Anthony Atala - Winston-Salem NC, US

CHILDREN'S MEDICAL CENTER CORPORATION - Boston MA

A61F 2/02, A61K 35/12, A61P 13/12

424423, 424 937

The present invention provides methods and compositions for augmenting organ functions using small-scale matrix implants generated by seeding tissue-specific or undifferentiated cells onto a matrix materials (e.g., a wafer, sponge, or hydrogel). The seeded matrix composition can then be implanted and will develop into an organ-supplementing structure in vivo. Continued growth and differentiation of the seeded cells on the implanted matrix results in the formation of a primitive vascular system in the tissue. The primitive vascular system can then develop into a mature vascular system, and can also support the growth and development of additional cultured cell populations. The seeded matrix system can be used to introduce a variety of different cells and tissues in vivo.

2007005, Mar 15, 2007

Aug 21, 2006

11/507402

Anthony Atala - Winston Salem NC, US

Children's Medical Center Corporation - Boston MA

A61K 35/14, C12N 5/08

424093700, 435366000

The present invention provides methods and compositions for supplementing or replacing a damaged organ. The damaged organ to be supplemented or replaced in accordance with the present invention include, for example, kidney, heart, liver, spleen, pancreas, bladder, ureter and urethra. In one embodiment, the tissue-engineered construct of the invention has has at least the following characteristics: (a) differentiated cells on a three-dimensional biocompatible scaffold, wherein the differentiated cells originated from transferred pluripotent cells; and (b) at least one physiological function of the organ.

2012010, May 3, 2012

Jan 6, 2012

13/344644

Anthony Atala - Winston Salem NC, US

CHILDREN'S MEDICAL CENTER CORPORATION - Boston MA

A61K 9/00, A61P 35/00, A61K 35/12

424484, 424 937

The invention is directed to methods and devices for the reconstruction, repair, augmentation or replacement of laminarily organized luminal organs or tissue structures in a patient in need of such treatment. The device comprises a biocompatible synthetic or natural polymeric matrix shaped to conform to at least a part of the luminal organ or tissue structure with a first cell population on or in a first area and a second cell population such as a smooth muscle cell population in a second area of the polymeric matrix. The method involves grafting the device to an area in a patient in need of treatment. The polymeric matrix comprises a biocompatible and biodegradable material.

2006023, Oct 26, 2006

Apr 13, 2006

11/279671

James Yoo - Winston-Salem NC, US
Joel Stitzel - Winston-Salem NC, US
Anthony Atala - Winston-Salem NC, US
George Christ - Winston-Salem NC, US

C12N 5/08, C12N 5/06

424093700, 435325000, 435366000

A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

2007011, May 24, 2007

Nov 28, 2006

11/604918

Anthony Atala - Winston Salem NC, US
Paolo De Coppi - Santa Lucia di Piave, IT

Children's Medical Center Corporation - Boston MA

A61K 35/12, G01N 33/567, C12N 5/00, C12N 5/08

424093700, 435325000, 435366000, 435007200

The present invention is directed to pluripotent fetal stem cells derived from chorionic villus, amniotic fluid, and placenta and the methods for isolating, expanding and differentiating these cells, and their therapeutic uses such as manipulating the fetal stem cells by gene transfection and other means for therapeutic applications.

2010011, May 6, 2010

Nov 13, 2009

12/618338

Anthony Atala - Winston-Salem NC, US
James J. Yoo - Winston-Salem NC, US

A61K 35/23, C12N 5/071, A61P 13/12

424484, 435369, 424 937

Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro, and methods of use thereof. The cells may be provided in a three dimensional matrix for culturing in vitro and/or implanting in vivo. Methods of seeding cells onto the matrix are also provided.

7429490, Sep 30, 2008

Feb 7, 2005

11/052459

Anthony Atala - Winston Salem NC, US
James J. Yoo - Brookline MA, US

Children's Medical Center - Boston MA

C12N 5/06, C12N 5/08, A61F 2/04

435373, 435 11, 623 2364

The invention is directed to compositions and methods for reconstructing artificial female reproductive organs. The constructs and methods of the invention can be used for ameliorating congenital malformations and disorders of female reproductive tract using tissue engineered female reproductive organs, such as the uterus, vagina, cervix, and fallopian tubes. These tissue engineered female reproductive organs can be generated by perfusing cultured cell populations derived from cells of the female reproductive tissues, such as uterine, vaginal, cervical, fallopian tube epithelial cells as well as smooth muscle cells.