Inventors:
Amanda G. Paulovich - Seattle WA, US
Richard G. Ivey - Federal Way WA, US
International Classification:
G01N 33/566, G01N 33/53
Abstract:
In one aspect, the invention provides a kit for detecting the presence or amount of a target modified protein within a sample. The kit comprises (i) a capture reagent that specifically binds to a first epitope on the target protein; (ii) at least one detection reagent that specifically binds to a second epitope on the target protein; and (iii) at least one synthetic hybrid reference peptide comprising the first epitope and the second epitope from the target protein of interest, wherein the synthetic reference peptide is capable of simultaneously binding to both the capture reagent and the at least one detection reagent, and wherein at least one of the first and second epitopes comprises a modified amino acid residue.