ALFRED O FAYEMI
Medical Practice in White Plains, NY
License number
New Jersey 25MF00050900
Issued Date
Jan 11, 1982
Expiration Date
Dec 31, 2017
Category
Medical Examiners
Type
Bioanalytical Laboratory Dir
Address
Address
White Plains, NY
Professional information
Enzymatic Immunoassay
US Patent:
4454226, Jun 12, 1984
Filed:
Mar 17, 1982
Appl. No.:
6/358888
Inventors:
Majid Ali - Teaneck NJ
Donald Nalebuff - Oakland NJ
Alfred Fayemi - White Plains NY
Madhava P. Ramanarayanan - Teaneck NJ
Ricardo Mesa-Tejada - Briarcliff Manor NY
Donald Nalebuff - Oakland NJ
Alfred Fayemi - White Plains NY
Madhava P. Ramanarayanan - Teaneck NJ
Ricardo Mesa-Tejada - Briarcliff Manor NY
International Classification:
G01N 3354, C12N 996, C12N 1102, C12Q 128
US Classification:
435 7
Abstract:
An enzyme immunoassay for detecting an antigen in a biologic fluid or tissue which comprises contacting the fluid or tissue with an antibody specific for the antigen under binding conditions, at least one of the fluid or tissue and antibody having a solid component, contacting the resulting solid with a conjugate bindable with the antibody under binding conditions and determining the enzyme activity of the resulting solid phase is described. The conjugate is of peroxidase and an allergen, non-immunoglobulin protein or primary amino group containing drug having an average of 2-3 molecules of peroxidase per molecule of substance with an average molecular weight of about 30,000 daltons, prepared by reacting peroxidase previously treated with phenyl isothiocyanate and oxidized to form aldehyde groups with the substance to form a Schiff's base which is titrated with a reducing agent to form a stable conjugate.
Enzyme Immunoassay For Allergic Disorders
US Patent:
4256833, Mar 17, 1981
Filed:
Jan 8, 1979
Appl. No.:
6/001874
Inventors:
Majid Ali - Teaneck NJ
Alfred Fayemi - White Plains NY
Madhava P. Ramanarayanan - New York NY
Ricardo Mesa-Tejada - Briarcliff Manor NY
Alfred Fayemi - White Plains NY
Madhava P. Ramanarayanan - New York NY
Ricardo Mesa-Tejada - Briarcliff Manor NY
International Classification:
C12Q 166, C12N 1102, C12N 996
US Classification:
435 7
Abstract:
Horse radish peroxidase (HRP) is treated with phenyl isothiocyanate (PITC) to block the free amino groups on the enzyme. The PITC derivative of HRP is treated with periodate to oxidize the carbohydrate moiety on the enzyme, thus generating aldehyde groups. Gamma G globulin fraction (IgG) purified from an anti-human IgE serum is conjugated to the peroxidase-aldehyde by formation of a Schiff's base between the aldehyde group on the enzyme and the amino groups on the IgG. The Schiff's base is stabilized by reduction using the optimal amounts of sodium borohydride determined by tiration. A stable HRP-anti IgE IgG conjugate prepared thus is employed in a solid phase enzyme immunoassay for the detection of allergen specific IgE. The results of this assay can be used to determine a safe initial hypersensitization dosage level.