ALBERTO JOSE SABUCEDO, M.D.,PH.D.
Osteopathic Medicine at Aventura Blvd, Miami, FL

2006000, Jan 12, 2006

Aug 12, 2005

11/202909

Alberto Sabucedo - Miami FL, US
Kenneth Furton - Miami FL, US

G01N 33/53

435007200

A method of determining time of death is disclosed. Cardiac troponin I (cTnI) is the most specific marker for the determination of myocardial infarction (MI). Cardiac troponin I has a distinctive temporal degradation profile after death, which is key to its use as a time of death marker in forensic medicine. The method consists of sampling cardiac tissue, extracting a cardiac protein from a homogenate via methods which may include the magnetic microparticles or magnetic microparticles linked to anti-cTnI antibodies, eluting the proteins, separating proteins by electrophoresis, transferring by western blot the different bands of proteins to paper. A comparison/analysis of the concentration and kinetics of degradation of the protein at a given temperature(s) against known standards after time of death provides an accurate measurement of the time of death. The present invention is more accurate and reliable than the rudimentary time of death techniques currently used by medical examiners.

6165981, Dec 26, 2000

Mar 7, 1995

8/400158

Cathy Flaa - Norfolk VA
Alberto Sabucedo - Miami FL
Bruce Chin - Miami FL
Roger Bauer - Ft. Lauderdale FL

Dade Behring Inc. - Deerfield IL

A61K 3800, C07K 1400

514 21

Disclosed are compositions for stabilizing proteins and fragments of the proteins. The composition contains buffer, salt, reducing agents, chelating agents and stabilizing proteins. The composition may be used to prepare highly stable diagnostic calibrators or controls and is particularly useful for calibrators or controls for cardiac markers such as troponin.