Alan George Barbour
Physician in San Antonio, TX

6077515, Jun 20, 2000

Aug 13, 1996

8/696372

Alan G. Barbour - San Antonio TX
Virgilio G. Bundoc - Newbury Park CA
Adriadna Sadziene - San Antonio TX

The University of Texas System, Board of Regents - Austin TX

A61K 3900, A61K 4500, A01N 6300, C12N 100

4242341

This invention relates to flagella-less strains of Borrelia and to novel methods for use of the microorganisms as vaccines and in diagnostic assays. Although a preferred embodiment of the invention is directed to Borrelia burgdorferi, the present invention encompasses flagella-less strains of other microorganisms belonging to the genus Borrelia. Accordingly, with the aid of the disclosure, flagella-less mutants of other Borrelia species, e. g. , B. coriacei, which causes epidemic bovine abortion, B. anserina, which causes avian spirochetosis, and B. recurrentis and other Borrelia species causative of relapsing fever, such as Borrelia hermsii, Borrelia turicatae, Borrelia duttoni, Borrelia persica, and Borrelia hispanica, can be prepared and used in accordance with the present invention and are within the scope of the invention. Therefore, a preferred embodiment comprises a composition of matter comprising a substantially pure preparation of a strain of a flagella-less microorganism belonging to the genus Borrelia.

5585102, Dec 17, 1996

Sep 20, 1993

8/124290

Alan G. Barbour - San Antonio TX
Virgilio G. Bundoc - Newbury Park CA
Adriadna Sadziene - San Antonio TX

Board of Regents, The University of Texas System - Austin TX

C12N 100, C12N 136, A61K 3902, C12Q 102

4242341

This invention relates to flagella-less strains of Borrelia to novel methods for use of the microorganisms as vaccines and in diagnostic assays. Although a preferred embodiment of the invention is directed to Borrelia burgdorferi, the present invention encompasses flagella-less strains of other microorganisms belonging to the genus Borrelia. Accordingly, with the aid of the disclosure, flagella-less mutants of other Borrelia species, e. g. , B. coriacei, which causes epidemic bovine abortion, B. anserina, which causes avian spirochetosis, and B. recurrentis and other Borrelia species causative of relapsing fever, such as Borrelia hermsii, Borrelia turicatae, Borrelia duttoni, Borrelia persica, and Borrelia hispanica, can be prepared and used in accordance with the present invention and are within the scope of the invention. Therefore, a preferred embodiment comprises a composition of matter comprising a substantially pure preparation of a strain of a flagella-less microorganism belonging to the genus Borrelia.

5436000, Jul 25, 1995

Jan 11, 1991

7/641143

Alan G. Barbour - San Antonio TX
Virgilio Bundoc - San Antonio TX

University of Texas System - Austin TX

A61K 3902, A61K 4800, C12N 136

424 932

This invention relates to flagella-less strains of Borrelia and to novel methods for use of the microorganisms as vaccines and in diagnostic assays. Although a preferred embodiment of the invention is directed to Borrelia burgdorferi, the present invention encompasses flagella-less strains of other microorganisms belonging to the genus Borrelia. Accordingly, with the aid of the disclosure, flagella-less mutants of other Borrelia species, e. g. , B. coriacei, which causes epidemic bovine abortion, B. anserina, which causes avian spirochetosis, and B. recurrentis and other Borrelia species causative of relapsing fever, such as Borrelia hermsii, Borrelia turicatae, Borrelia duttoni, Borrelia persica, and Borrelia hispanica, can be prepared and used in accordance with the present invention and are within the scope of the invention. Therefore, a preferred embodiment comprises a composition of matter comprising a substantially pure preparation of a strain of a flagella-less microorganism belonging to the genus Borrelia.

6068842, May 30, 2000

Jun 6, 1995

8/471733

Sven Bergstrom - Umea, SE
Alan George Barbour - San Antonio TX

Symbicom AB - Ulmea

A61K 3900, A61K 3902, C12P 2106, C12N 1500

4241841

The present invention relates to nucleic acid molecules, polypeptides encoded by the same, antibodies directed thereto and a method of preparing such polypeptides including: (a) inserting an isolated DNA molecule coding for a polypeptide which is immunoreactive with a 66 kDa polypeptide derived from Borrelia garinii IP90 into an expression vector; (b) transforming a host organism or cell with the vector; (c) culturing the transformed host cell under suitable conditions; and (d) harvesting the polypeptide. The isolated DNA molecule is preferably at least 10 nucleotides in length, and the method may optionally include subjecting the polypeptide to post-translational modification. The host cell can be a bacterium, a yeast, a protozoan, or a cell derived from a multicellular organism such as a fungus, an insect cell, a plant cell, or a mammalian cell.

5246844, Sep 21, 1993

Oct 22, 1991

7/781355

Steven J. Norris - Houston TX
Alan G. Barbour - San Antonio TX

Board of Regents, The University of Texas System - Austin TX

C12N 1500, C12N 1503, C12N 1531

4351723

The invention relates to a DNA segment encoding a Borrelia burgdorferi antigenic polypeptide. The invention also relates to a purified 30 kDa polypeptide isolated from a virulent strain of B. burgdorferi and to epitopic segments of the polypeptide with immunogenic potential. The 30 kDa protein provides a route for the development of immunodiagnostics for Lyme disease and related disorders. The 30 kDa protein and related amino acid and DNA sequences may also be used for the immunization, for the detection of B. burgdorferi in human or animal tissues or body fluids, and also for the generation of specific antibodies for use in diagnosis, epidemiology, and prevention of Lyme disease.

6090586, Jul 18, 2000

Jun 6, 1995

8/468878

Sven Bergstrom - Umea, SE
Alan George Barbour - San Antonio TX

Symbicom AB - Umea

A61K 3900, A61K 3902, C12P 2106, C12N 1500

435 693

The present invention relates to nucleic acid molecules, polypeptides encoded by the same, antibodies directed thereto and a method of preparing such polypeptides including: (a) inserting an isolated DNA molecule coding for a polypeptide which is immunoreactive with a 66 kDa polypeptide derived from Borrelia garinii IP90 into an expression vector; (b) transforming a host organism or cell with the vector; (c) culturing the transformed host cell under suitable conditions; and (d) harvesting the polypeptide. The isolated DNA molecule is preferably at least 10 nucleotides in length, and the method may optionally include subjecting the polypeptide to post-translational modification. The host cell can be a bacterium, a yeast, a protozoan, or a cell derived from a multicellular organism such as a fungus, an insect cell, a plant cell, or a mammalian cell.

5777095, Jul 7, 1998

Oct 26, 1993

8/137175

Alan George Barbour - San Antonio TX
Sven Bergstrom - Umea, SE
Lennart Hansson - Umea, SE

Symbicom Aktiebolag - Umea

C07H 2102, C07H 2104, C12Q 168, C12P 1934

536 237

Disclosed and claimed are isolated DNA molecules consisting of nucleotide sequences encoding or priming for ospA and/or ospB of various B. burgdorferi or portions thereof and methods of making and using the same.

6083722, Jul 4, 2000

Jun 6, 1995

8/471019

Sven Bergstrom - Umea, SE
Alan G. Barbour - San Antonio TX
Louis A. Magnarelli - Durham CT

Symbicom AB - Ume.ang.

C12P 2106, C12P 1934, C07H 2104

435 693

Disclosed and claimed are Lyme disease vaccines and methods for making and using them. The vaccines include a vector containing DNA encoding OspA or an immunogenic fragment thereof or such DNA encoding OspA or an immunogenic fragment thereof which has been modified by substitution, addition, insertion or deletion of one or more nucleotides, whereby the DNA when expressed results in OspA, or an immunogenic fragment thereof, or a polypeptide having the immunological activity of OspA or the fragment thereof.

5523089, Jun 4, 1996

Jun 22, 1993

8/079601

Sven Bergstrom - Umea, SE
Alan G. Barbour - San Antonio TX
Louis A. Magnarelli - Durham CT

Symbicom Aktiebolag

A61K 3900, A61K 3902

4242621

B fraction of Borrelia burgdorferi, methods for preparing the B fraction, and compositions containing the B fraction, are disclosed and claimed.

6203798, Mar 20, 2001

Jun 6, 1995

8/470627

Sven Bergstrom - Umea, SE
Alan G. Barbour - San Antonio TX
Louis A. Magnarelli - Durham CT

Symbicom Aktiebolag - Umea

A61K 3902, A61K 3938, G01N 3353, C07K 100

4242341

Disclosed and claimed are: substantially pure lipidated OspA protein, compositions containing substantially pure lipidated OspA protein, immunogenic fragments of OspA, compositions containing immunogenic fragments of OspA, polypeptides containing an immunogenic fragment or epitopic region of OspA, and methods for making and using such proteins, fragments, and polypeptides.