AKHILESH PANDEY
Pilots at Edward Hl Rd, Ellicott, MD
License number
Maryland A4024410
Issued Date
Dec 2016
Expiration Date
Dec 2017
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
6134 Edward Hill Rd, Ellicott, MD 21043
Professional information
Mutant Proteins As Cancer-Specific Biomarkers
US Patent:
2014005, Feb 20, 2014
Filed:
Jan 17, 2012
Appl. No.:
13/980005
Inventors:
Bert Vogelstein - Baltimore MD, US
Qing Wang - Parkville MD, US
Akhilesh Pandey - Ellicott city MD, US
Kenneth W. Kinzler - Baltimore MD, US
Nickolas Papadopoulos - Towson MD, US
Qing Wang - Parkville MD, US
Akhilesh Pandey - Ellicott city MD, US
Kenneth W. Kinzler - Baltimore MD, US
Nickolas Papadopoulos - Towson MD, US
Assignee:
THE JOHNS HOPKINS UNIVERSITY - Baltimore MD
International Classification:
G01N 33/68
US Classification:
435 23
Abstract:
Altered protein products resulting from somatic mutations are directly identified and quantified by mass spectrometry. The peptides expressed from normal and mutant alleles are detected by Selected Reaction Monitoring (SRM) of their productions using a triple quadrupole mass spectrometer. As a prototypical example of this approach, we quantify the number and fraction of mutant Ras protein present in cancer cell lines. There were an average of 1.3 million molecules of Ras protein per cell and the ratio of mutant to normal Ras proteins ranged from 0.49 to 5.6. Similarly, we detected and quantified mutant Ras proteins in clinical specimens such as colorectal and pancreatic tumor tissues as well as in pre-malignant pancreatic cyst fluids. These methods are useful for diagnostic applications.