Inventors:
Victor J. Dzau - Newton MA
Richard E. Pratt - Newton MA
Michael J. Mann - Newton MA
Afshin Ehsan - Boston MA
Daniel P. Griese - Boston MA
Assignee:
The Bringham and Womens Hospital - Boston MA
International Classification:
A61F 206
US Classification:
623 14, 623 11, 623 113, 623 139, 623 141, 623 142, 623 143, 623924, 623926, 606191, 606192, 606194, 606195, 606198
Abstract:
A method for implanting cells onto a prosthesis includes the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 m, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 m, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. A method for obtaining an endothelial cell culture from a blood sample involved:(a) obtaining a sample for mononuclear cells from a blood sample; and (b) culturing the sample of mononuclear cells, without further cell separation, on a cell adhesive polymer-coated solid support in the presence of endothelial growth factors.