DR. ADRIAN M. DI BISCEGLIE, M.D.
Osteopathic Medicine at Vista Ave, Saint Louis, MO
License number
Missouri 105308
Category
Osteopathic Medicine
Type
Gastroenterology
Address
Address
3660 Vista Ave, Saint Louis, MO 63110
Phone
(314) 577-8764
(314) 577-8125 (Fax)
(314) 977-6828
(314) 977-6777 (Fax)
(314) 577-8125 (Fax)
(314) 977-6828
(314) 977-6777 (Fax)
Professional information
Compositions And Methods For Amplification And Cloning Of Near Full-Length Viral Genome Samples
US Patent:
7786294, Aug 31, 2010
Filed:
May 31, 2007
Appl. No.:
11/756250
Inventors:
Xiaofeng Fan - St. Louis MO, US
Adrian M. Di Bisceglie - Ladue MO, US
Adrian M. Di Bisceglie - Ladue MO, US
Assignee:
Saint Louis University - Saint Louis MO
International Classification:
C07H 21/00
US Classification:
536 253, 536 2531, 536 2532, 536 2533, 536 2534
Abstract:
A method of producing a polydeoxyribonucleotide molecule by reverse transcriptase polymerase chain reaction wherein the polydeoxyribonucleotide molecule has a length of greater than 5,000 base-pairs is disclosed. The method involves combining two reverse transcriptases followed by two protocols of polymerase chain reaction. This method enable the amplification of large DNAs, such as viruses, from a sample while preserving genetic diversity of the large DNA.